Biffle led 34 of the 80 laps with pole-sitter Matt Kenseth dominated the start of the race before the first green-flag pit stop on Lap 40.
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Daytona International Speedway, 1801 W International Speedway Blvd, Daytona Beach, FL 32114, USA
Josh Wise and the No. 26 Ford took over the lead after the pit stop but that advantage disappeared when Martin Truex Jr. took the top spot. His lead was short-lived as Biffle maneuvered his No. 16 Ford to the front of the pack followed by Kenseth.
Kasey Kahne was third followed by Ryan Newman and Dale Earnhardt Jr. to finish out the top 5.
Matt Kenseth led after 100 miles.
Kenseth started from the pole for tonight's race and led all 40 laps so far.
The Roush Fenway Racing star was looking to become the first driver since Bobby Allison in 1982 to win both NASCAR races in the same season. The 40-year-old Kenseth won the Daytona 500 earlier this year.
It was the first pole of the season for Kenseth, who started the race as the Sprint Cup points leaders.
Greg Biffle, who started fourth, trailed Kenseth in second place.
Bill Elliott and the No. 50 Chevy was in third place followed by Carl Edwards and Ryan Newman to round out the top five.
Newman's teammate, Tony Stewart, originally qualified second but his time was disallowed after his No. 14 Chevy failed a post-qualifying inspection when NASCAR found an open cooling hose in his cockpit. He was pushed to the rear of the field for Saturday's race.